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Human TAF5 Recombinant Protein (N-His) (HD242012)

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Overview
Catalog No.HD242012
Description
Recombinant Human TAF5 Protein, N-His (HD242012) expressed in E. coli, spanning Leu458-Gln800. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ15542
Protein lengthLeu458-Gln800
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 40.30 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesTAF(II)100, TAF2D, TAF5, TAFII-100, TAFII100, Transcription initiation factor TFIID 100 kDa subunit, Transcription initiation factor TFIID subunit 5
Background

Transcription initiation factor TFIID subunit 5 is a ~86 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TAF5 is involved in two modules of TFIID, in TFIID-A together with TAF3 and TBP, and in TFIID-B with TAF8.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Dubrovskaya, V. et al. (1996) The EMBO journal 15, 3702-12. PMID: 8758937
3. Tanese, N. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 13611-6. PMID: 8942982
4. Tao, Y. et al. (1997) The Journal of biological chemistry 272, 6714-21. PMID: 9045704
Note For research use only
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Formula
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