Anti-Human TNFa/TNF-alpha Recombinant Antibody (SAA2236) (HF879093)

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Spec:

100ug
1mg

Number:

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References
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SDS

Overview

Catalog No.

HF879093

Description

Anti-Human TNFa/TNF-alpha Antibody (SAA2236) [SAA2236] (HF879093) is a rabbit monoclonal antibody detecting TNF-alpha in ELISA. Suitable for Human.

Highlights

●Rabbit Monoclonal — High specificity, affinity, and excellent lot-to-lot consistency.

Species reactivity

Human

Applications

ELISA

Host species

Rabbit

Isotype

IgG

Clone ID

SAA2236

Clonality

Monoclonal

Target

TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01375

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. TNFa is the therapeutic target of adalimumab (Humira).

1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208

2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952

3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918

4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human TNFa/TNF-alpha Antibody (SAA2236)

References

Datasheet

Anti-Human TNFa/TNF-alpha Recombinant Antibody (SAA2236).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TNFa/TNF-alpha Recombinant Antibody (SAA2236) (HF879093)

Overview

Images

References

Datasheet

SDS

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