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Anti-Human TOM1 Polyclonal Antibody (HF504014)

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Overview
Catalog No.HF504014
Description
Anti-Human TOM1 Polyclonal Antibody (HF504014) is a rabbit polyclonal antibody detecting TOM1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TOM1 (Met1-Gly148).
Target Target of Myb1 membrane trafficking protein, Target of Myb protein 1, TOM1
Purification Purified by antigen affinity column.
Accession O60784
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Target of Myb1 membrane trafficking protein (TOM1) is a ~53 kDa protein. Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways. Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures. Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes. MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN. Binds to polyubiquitinated proteins via its GAT domain.

1. Yamakami, M. et al. (2003) The Journal of biological chemistry 278, 52865-72. PMID: 14563850
2. Katoh, Y. et al. (2004) The Journal of biological chemistry 279, 24435-43. PMID: 15047686
3. Tumbarello, DA. et al. (2012) Nature cell biology 14, 1024-35. PMID: 23023224
4. Boal, F. et al. (2015) Journal of cell science 128, 815-27. PMID: 25588840
5. Xiao, S. et al. (2015) Structure (London, England : 1993) 23, 1910-1920. PMID: 26320582
6. Hu, S. et al. (2019) Nature communications 10, 3459. PMID: 31371777
Note For research use only.
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Datasheet
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Formula
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Anti-Human TOM1 Polyclonal Antibody

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