Human TOM1 Recombinant Protein (N-His) (HF504012)

Name: Human TOM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF504012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF504012
Description	Recombinant Human TOM1 Protein, N-His (HF504012) expressed in E. coli, spanning Met1-Gly148. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60784
Protein length	Met1-Gly148
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.60 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Target of Myb1 membrane trafficking protein, Target of Myb protein 1, TOM1
Background	Target of Myb1 membrane trafficking protein (TOM1) is a ~53 kDa protein. Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pathways. Acts as a MYO6/Myosin VI adapter protein that targets MYO6 to endocytic structures. Together with MYO6, required for autophagosomal delivery of endocytic cargo, the maturation of autophagosomes and their fusion with lysosomes. MYO6 links TOM1 with autophagy receptors, such as TAX1BP1; CALCOCO2/NDP52 and OPTN. Binds to polyubiquitinated proteins via its GAT domain. 1. Yamakami, M. et al. (2003) The Journal of biological chemistry 278, 52865-72. PMID: 14563850 2. Katoh, Y. et al. (2004) The Journal of biological chemistry 279, 24435-43. PMID: 15047686 3. Tumbarello, DA. et al. (2012) Nature cell biology 14, 1024-35. PMID: 23023224 4. Boal, F. et al. (2015) Journal of cell science 128, 815-27. PMID: 25588840 5. Xiao, S. et al. (2015) Structure (London, England : 1993) 23, 1910-1920. PMID: 26320582 6. Hu, S. et al. (2019) Nature communications 10, 3459. PMID: 31371777
Note	For research use only.

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SDS-PAGE for Recombinant Human TOM1 Protein, N-His

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Datasheet

Human TOM1 Recombinant Protein (N-His).pdf

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Human TOM1 Recombinant Protein (N-His) (HF504012)

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