Anti-Human TRIB3 Polyclonal Antibody (HA514014)

Name: Anti-Human TRIB3 Polyclonal Antibody
Brand: abinScience
SKU: HA514014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA514014

Description

Anti-Human TRIB3 Polyclonal Antibody (HA514014) is a rabbit polyclonal antibody detecting TRIB3 in ELISA, IHC, WB. Suitable for Bovine and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human TRIB3 (Ser66-Gln317).

Target

TRIB3

Purification

Purified by antigen affinity column.

Accession

Q96RU7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, TRIB3, C20orf97, NIPK, SKIP3, TRB3

Background

Tribbles homolog 3 (TRIB3) is a ~39 kDa protein. Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation.

1. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988

2. Ord, D. et al. (2005) Biochemical and biophysical research communications 330, 210-8. PMID: 15781252

3. Wu, M. et al. (2003) The Journal of biological chemistry 278, 27072-9. PMID: 12736262

4. Kiss-Toth, E. et al. (2004) The Journal of biological chemistry 279, 42703-8. PMID: 15299019

5. Aynaud, MM. et al. (2012) The Journal of biological chemistry 287, 39182-92. PMID: 22977230

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TRIB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: LO2 cell lysate
Lane 2: PC-3 cell lysate

References

Datasheet

Anti-Human TRIB3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TRIB3 Polyclonal Antibody (HA514014)

Overview

Images

References

Datasheet

SDS

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