Human TRIB3 Recombinant Protein (N-His) (HA514012)

Name: Human TRIB3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA514012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA514012
Description	Recombinant Human TRIB3 Protein, N-His (HA514012) expressed in E. coli, spanning Ser66-Gln317. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96RU7
Protein length	Ser66-Gln317
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.45 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, TRIB3, C20orf97, NIPK, SKIP3, TRB3
Background	Tribbles homolog 3 is a ~39 kDa protein. Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. 1. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988 2. Ord, D. et al. (2005) Biochemical and biophysical research communications 330, 210-8. PMID: 15781252 3. Wu, M. et al. (2003) The Journal of biological chemistry 278, 27072-9. PMID: 12736262 4. Kiss-Toth, E. et al. (2004) The Journal of biological chemistry 279, 42703-8. PMID: 15299019 5. Aynaud, MM. et al. (2012) The Journal of biological chemistry 287, 39182-92. PMID: 22977230
Note	For research use only.

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SDS-PAGE for Recombinant Human TRIB3 Protein, N-His

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Datasheet

Human TRIB3 Recombinant Protein (N-His).pdf

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Human TRIB3 Recombinant Protein (N-His) (HA514012)

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