Anti-Human TRIM28 IP Recombinant Antibody (SAA0249) (HC420013)

Name: Anti-Human TRIM28 IP Recombinant Antibody (SAA0249)
Brand: abinScience
SKU: HC420013
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC420013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0249

Clonality

Monoclonal

Target

TIF1-beta, Tripartite motif-containing protein 28, KRAB-associated protein 1, TRIM28, RING-type E3 ubiquitin transferase TIF1-beta, KRAB-interacting protein 1, KRIP-1, E3 SUMO-protein ligase TRIM28, RNF96, Nuclear corepressor KAP-1, TIF1B, Transcription intermediary factor 1-beta, KAP1, KAP-1, RING finger protein 96

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13263

RRID

Anti-Human TRIM28 IP Antibody (SAA0249) (abinScience Cat# HC420013, RRID:AB_3729783)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Transcription intermediary factor 1-beta (TRIM28) is a ~88 kDa protein. Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1.

1. Huang, C. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 4470-3. PMID: 23543754

2. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

3. Valle-García, D. et al. (2016) Epigenetics 11, 398-414. PMID: 27029610

Note

For research use only.

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