Anti-Human TRIM28 Polyclonal Antibody (HC420014)

Name: Anti-Human TRIM28 Polyclonal Antibody
Brand: abinScience
SKU: HC420014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC420014

Description

Anti-Human TRIM28 Polyclonal Antibody (HC420014) is a rabbit polyclonal antibody detecting TRIM28 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRIM28 (Ser50-Arg136).

Target

TIF1-beta, Tripartite motif-containing protein 28, KRAB-associated protein 1, TRIM28, RING-type E3 ubiquitin transferase TIF1-beta, KRAB-interacting protein 1, KRIP-1, E3 SUMO-protein ligase TRIM28, RNF96, Nuclear corepressor KAP-1, TIF1B, Transcription intermediary factor 1-beta, KAP1, KAP-1, RING finger protein 96

Purification

Purified by antigen affinity column.

Accession

Q13263

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription intermediary factor 1-beta (TRIM28) is a ~88 kDa protein. Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1.

1. Huang, C. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 4470-3. PMID: 23543754

2. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306

3. Valle-García, D. et al. (2016) Epigenetics 11, 398-414. PMID: 27029610

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human TRIM28 polyclonal antibody at 1ug/mL on recombinant Human TRIM28 (Catalog No. HC420012).

References

Datasheet