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Anti-Human TSPAN4 Polyclonal Antibody (HD423014)

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Overview
Catalog No.HD423014
Description
Anti-Human TSPAN4 Polyclonal Antibody (HD423014) is a rabbit polyclonal antibody detecting TSPAN4. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TSPAN4 (Tyr107-Asn201).
Target NAG-2, NAG2, Novel antigen 2, TM4SF7, TSPAN4, Tetraspanin-4, Transmembrane 4 superfamily member 7, Tspan-4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O14817
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Tetraspanin-4 (TSPAN4) is a ~26 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays an essential role in migrasome formation and migration on retracting fibers at the rear end of migrating cells. Migrasomes are cellular organelles that form as large vesicle-like structures on retraction fibers of migrating cells. Mechanistically, acts as a membrane curvature sensor and participates in stabilizing the migrasome structure in a late stage of biogenesis. May also play a regulatory role for the histamine H4 receptor/HRH4 without affecting histamine binding to HRH4 or signaling.

1. Huang, Y. et al. (2019) Nature cell biology 21, 991-1002. PMID: 31371828
2. Dharan, R. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2208993119. PMID: 36252000
3. Dharan, R. et al. (2023) Nature communications 14, 1037. PMID: 36823145
4. Ma, X. et al. (2021) Biomolecules 11. PMID: 34439793
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human TSPAN4 Polyclonal Antibody

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