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Anti-Human VAMP8 Polyclonal Antibody (HP094014)

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Overview
Catalog No.HP094014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human VAMP8 (Glu2-Lys75).
Target VAMP8, EDB, Endobrevin, VAMP-8, Vesicle-associated membrane protein 8
Purification Purified by antigen affinity column.
Accession Q9BV40
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vesicle-associated membrane protein 8 (VAMP8) is a ~11 kDa protein. SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells.

1. Itakura, E. et al. (2012) Cell 151, 1256-69. PMID: 23217709
2. Diao, J. et al. (2015) Nature 520, 563-6. PMID: 25686604
3. Polgár, J. et al. (2002) Blood 100, 1081-3. PMID: 12130530
4. van Tol, S. et al. (2020) Journal of virology 94. PMID: 31694946
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Anti-Human VAMP8 Polyclonal Antibody

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