Human VAMP8 Recombinant Protein (N-His-SUMO & C-Strep) (HP094012)

Name: Human VAMP8 Recombinant Protein (N-His-SUMO & C-Strep)
Brand: abinScience
SKU: HP094012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP094012
Description	Recombinant Human VAMP8 Protein, N-His-SUMO & C-Strep (HP094012) expressed in E. coli, spanning Glu2-Lys75. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BV40
Protein length	Glu2-Lys75
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	VAMP8, EDB, Endobrevin, VAMP-8, Vesicle-associated membrane protein 8
Background	Vesicle-associated membrane protein 8 (VAMP8) is a ~11 kDa protein. SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells. 1. Itakura, E. et al. (2012) Cell 151, 1256-69. PMID: 23217709 2. Diao, J. et al. (2015) Nature 520, 563-6. PMID: 25686604 3. Polgár, J. et al. (2002) Blood 100, 1081-3. PMID: 12130530 4. van Tol, S. et al. (2020) Journal of virology 94. PMID: 31694946
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human VAMP8 Protein, N-His-SUMO & C-Strep

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Datasheet

Human VAMP8 Recombinant Protein (N-His-SUMO & C-Strep).pdf

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Human VAMP8 Recombinant Protein (N-His-SUMO & C-Strep) (HP094012)

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Datasheet

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