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Anti-Human WNT3 Polyclonal Antibody (HC749014)

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Overview
Catalog No.HC749014
Description
Anti-Human WNT3 Polyclonal Antibody (HC749014) is a rabbit polyclonal antibody detecting WNT3. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human WNT3 (Gly50-Lys355).
Target INT4, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, WNT3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P56703
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Proto-oncogene Wnt-3 (WNT3) is a ~39 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development.

1. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
2. Niemann, S. et al. (2004) American journal of human genetics 74, 558-63. PMID: 14872406
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human WNT3 Polyclonal Antibody

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