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Anti-Human ZEB1 Polyclonal Antibody (HW705014)

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Anti-Human ZEB1 Polyclonal Antibody
Anti-Human ZEB1 Polyclonal Antibody
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Overview
Catalog No.HW705014
Description
Anti-Human ZEB1 Polyclonal Antibody (HW705014) is a rabbit polyclonal antibody detecting ZEB1 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ZEB1 (Ser583-Glu643).
Target Transcription factor 8, NIL-2-A zinc finger protein, Zinc finger E-box-binding homeobox 1, ZEB1, Negative regulator of IL2, TCF-8, TCF8, AREB6
Purification Purified by antigen affinity column.
Accession P37275
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Zinc finger E-box-binding homeobox 1 (ZEB1) is a ~124 kDa protein. Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1.

Note For research use only.
Images
  • Anti-Human ZEB1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ZEB1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ZEB1 Polyclonal Antibody

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