Anti-IL15 Polyclonal Antibody (HW541014)

Name: Anti-IL15 Polyclonal Antibody
Brand: abinScience
SKU: HW541014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
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Overview

Catalog No.

HW541014

Description

Anti-IL15 Polyclonal Antibody (HW541014) is a rabbit polyclonal antibody detecting IL15 in ELISA, IHC, WB. Suitable for Human, Macaca mulatta, and Macaca thibetana.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Chlorocebus aethiops, Macaca mulatta, Macaca thibetana

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL15 (Asn49-Ser162).

Target

IL15, IL-15, Interleukin-15

Purification

Purified by antigen affinity column.

Accession

P40933

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-15 (IL15) is a ~18 kDa protein. Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5.

1. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770

2. Grabstein, KH. et al. (1994) Science (New York, N.Y.) 264, 965-8. PMID: 8178155

3. Badolato, R. et al. (1997) Blood 90, 2804-9. PMID: 9326248

4. Musso, T. et al. (1999) Blood 93, 3531-9. PMID: 10233906

5. Ring, AM. et al. (2012) Nature immunology 13, 1187-95. PMID: 23104097

6. Giri, JG. et al. (1994) The EMBO journal 13, 2822-30. PMID: 8026467

7. Johnston, JA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8705-9. PMID: 7568001

Note

For research use only.

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