Anti-Human IL36 alpha/IL-1F6 Polyclonal Antibody (HV454014)

Name: Anti-Human IL36 alpha/IL-1F6 Polyclonal Antibody
Brand: abinScience
SKU: HV454014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV454014

Description

Anti-IL36A/IL-1F6 Polyclonal Antibody (HV454014) is a rabbit polyclonal antibody detecting IL-1F6 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human IL36A/IL-1F6 (Met1-Phe158).

Target

IL1F6, IL-1 epsilon, IL1E, IL36A, Interleukin-1 family member 6, FIL1 epsilon, FIL1E, IL-1F6, Interleukin-36 alpha, Interleukin-1 epsilon

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

Q9UHA7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-36 alpha (IL36A/IL-1F6) is a ~17 kDa protein. Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of pro-inflammatory cytokines such as TNF, IL-8 and IL-6. In cultured monocytes up-regulates expression of IL-1A, IL-1B and IL-6.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL36A polyclonal antibody at 1μg/ml on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: Mouse lung lysate
Lane 4: Rat brian lysate
Lane 5: Rat lung lysate

References

Datasheet

Anti-Human IL36 alpha/IL-1F6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IL36 alpha/IL-1F6 Polyclonal Antibody (HV454014)

Overview

Images

References

Datasheet

SDS