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Anti-Influenza A virus Matrix protein 2/M2e Polyclonal Antibody (VK736044)

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Overview
Catalog No.VK736044
Description
Anti-Influenza A virus (H7N9) M2e Polyclonal Antibody (VK736044) is a rabbit polyclonal antibody detecting matrix protein 2 [Influenza A virus (A/Hangzhou/1/2013(H7N9))]. Suitable for A/Hangzhou/1/2013(H7N9.
Species reactivityInfluenza A virus (H7N9)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Influenza A virus (H7N9) M2e (Met1-Pro25).
Target matrix protein 2 [Influenza A virus (A/Hangzhou/1/2013(H7N9))], M2, Influenza Matrix protein (M2); pfam00599
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession AGI60299.2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein 2 is a ~11 kDa protein. Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Influenza A virus Matrix protein 2/M2e Polyclonal Antibody

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