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Influenza A virus (H7N9) Matrix protein 2/M2e Recombinant Protein (N-GST & C-His) (VK736052)

Influenza A virus (H7N9) Matrix protein 2/M2e Recombinant Protein (N-GST & C-His)
Influenza A virus (H7N9) Matrix protein 2/M2e Recombinant Protein (N-GST & C-His)
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  • 100ug
  • 1mg
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  • Overview

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  • References

  • Datasheet

  • SDS

Overview
Catalog No.VK736052
Description
Recombinant Influenza A virus Matrix protein 2, N-GST & C-His (VK736052) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionAGI60299.2
Protein lengthMet1-Pro25
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesInfluenza A virus (H7N9)
Nature Recombinant
Endotoxin level Remove endotoxin and sterilization is required for cell assay.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 30.90 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein 2 is a ~11 kDa protein. Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway.

Note For research use only
Images
  • Influenza A virus (H7N9) Matrix protein 2/M2e Recombinant Protein (N-GST & C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Influenza A virus (H7N9) M2e Protein, N-GST & C-His

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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