Anti-LGALS9 Polyclonal Antibody (HT173014)

Name: Anti-LGALS9 Polyclonal Antibody
Brand: abinScience
SKU: HT173014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT173014

Description

Anti-LGALS9 Polyclonal Antibody (HT173014) is a rabbit polyclonal antibody detecting LGALS9 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LGALS9 (Met1-Gln148).

Target

Ecalectin, LGALS9, Galectin-9, Gal-9, Tumor antigen HOM-HD-21

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O00182

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Galectin-9 (Gal-9) is a ~39 kDa protein. Binds galactosides. Has high affinity for the Forssman pentasaccharide. Ligand for HAVCR2/TIM3. Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death. Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

1. Nagae, M. et al. (2008) Journal of molecular biology 375, 119-35. PMID: 18005988

2. Zhu, C. et al. (2005) Nature immunology 6, 1245-52. PMID: 16286920

3. Bi, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10650-5. PMID: 21670307

4. Gieseke, F. et al. (2013) European journal of immunology 43, 2741-9. PMID: 23817958

5. Mengshol, JA. et al. (2010) PloS one 5, e9504. PMID: 20209097

6. Dai, SY. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 2974-81. PMID: 16116184

7. Kojima, R. et al. (2014) PloS one 9, e86106. PMID: 24465902

9. Golden-Mason, L. et al. (2013) Journal of virology 87, 4835-45. PMID: 23408620

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LGALS9 polyclonal antibody at 1ug/mL on recombinant LGALS9 (Catalog No. HT173012).

References

Datasheet

Anti-LGALS9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-LGALS9 Polyclonal Antibody (HT173014)

Overview

Images

References

Datasheet

SDS

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