Anti-Human LPL/LIPD Polyclonal Antibody (HY363014)

Name: Anti-Human LPL/LIPD Polyclonal Antibody
Brand: abinScience
SKU: HY363014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY363014

Description

Anti-Human LPL/LIPD Polyclonal Antibody (HY363014) is a rabbit polyclonal antibody detecting LPL/LIPD in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human LPL (His55-Glu316).

Target

Phospholipase A1, LIPD, Lipoprotein lipase, LPL

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P06858

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lipoprotein lipase (LPL/LIPD) is a ~53 kDa protein. Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.

1. Lutz, EP. et al. (2001) The Journal of clinical investigation 107, 1183-92. PMID: 11342582

2. Pingitore, P. et al. (2016) Journal of clinical lipidology 10, 816-823. PMID: 27578112

3. Weinstock, PH. et al. (1995) The Journal of clinical investigation 96, 2555-68. PMID: 8675619

4. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167

5. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706

6. Goulbourne, CN. et al. (2014) Cell metabolism 19, 849-60. PMID: 24726386

7. Allan, CM. et al. (2017) Journal of lipid research 58, 216-225. PMID: 27811232

Note

For research use only.

Images

References

Datasheet

Anti-Human LPL/LIPD Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human LPL/LIPD Polyclonal Antibody (HY363014)

Overview

Images

References

Datasheet

SDS