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Anti-MASP2 Polyclonal Antibody (HT020014)

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Overview
Catalog No.HT020014
Description
Anti-MASP2 Polyclonal Antibody (HT020014) is a rabbit polyclonal antibody detecting MASP2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MASP2 (Thr287-Phe686).
Target Mannan-binding lectin serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MBL-associated serine protease 2, MASP2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00187
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mannan-binding lectin serine protease 2 (MASP2) is a ~75 kDa protein. Precursor of a serum protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface. MASP2 is cleaved and activated by MASP1 in response to lectin-binding to pathogen carbohydrates. Can activate prothrombin to thrombin.

1. Rossi, V. et al. (2001) The Journal of biological chemistry 276, 40880-7. PMID: 11527969
2. Héja, D. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 10498-503. PMID: 22691502
3. Degn, SE. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 3957-69. PMID: 22966085
4. Matsushita, M. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 165, 2637-42. PMID: 10946292
5. Kidmose, RT. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 15425-30. PMID: 22949645
6. Thiel, S. et al. (1997) Nature 386, 506-10. PMID: 9087411
7. Valenzuela Leon, PC. et al. (2025) Journal of medicinal chemistry 68, 4663-4671. PMID: 39924859
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MASP2 Polyclonal Antibody

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