Anti-MDM2 Polyclonal Antibody (HX174014)

Name: Anti-MDM2 Polyclonal Antibody
Brand: abinScience
SKU: HX174014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX174014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MDM2 (Asn3-Pro491).

Target

MDM2, Hdm2, p53-binding protein Mdm2, E3 ubiquitin-protein ligase Mdm2, RING-type E3 ubiquitin transferase Mdm2, Double minute 2 protein, Oncoprotein Mdm2

Purification

Purified by antigen affinity column.

Accession

Q00987

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase Mdm2 is a ~55 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein.

1. Liang, L. et al. (2018) Cell chemical biology 25, 761-774.e5. PMID: 29681526

2. Girnita, L. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 8247-52. PMID: 12821780

3. Li, M. et al. (2004) Molecular cell 13, 879-86. PMID: 15053880

4. Bernardi, R. et al. (2004) Nature cell biology 6, 665-72. PMID: 15195100

5. Brady, M. et al. (2005) Molecular and cellular biology 25, 545-53. PMID: 15632057

6. Sdek, P. et al. (2005) Molecular cell 20, 699-708. PMID: 16337594

7. Stevenson, LF. et al. (2007) The EMBO journal 26, 976-86. PMID: 17290220

8. Chen, D. et al. (2009) EMBO reports 10, 166-72. PMID: 19098711

9. Busso, CS. et al. (2009) Oncogene 28, 1616-25. PMID: 19219073

10. Li, DQ. et al. (2009) The Journal of biological chemistry 284, 34545-52. PMID: 19837670

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MDM2 polyclonal antibody at 1ug/mL on recombinant MDM2 (Catalog No. HX174012).

References

Datasheet

Anti-MDM2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MDM2 Polyclonal Antibody (HX174014)

Overview

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References

Datasheet

SDS

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