Anti-MECP2 Recombinant Antibody (N227/12) (HW353023)

Name: Anti-MECP2 Recombinant Antibody (N227/12)
Brand: abinScience
SKU: HW353023
Price: 196 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW353023

Description

Anti-MECP2 Recombinant Antibody (N227/12) (HW353023) is a mouse monoclonal antibody detecting MECP2 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N227/12

Target

MeCp2, MeCp-2 protein, Methyl-CpG-binding protein 2, MECP2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-MECP2 Antibody (N227/12) (abinScience Cat# HW353023, RRID:AB_3728260)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Methyl-CpG-binding protein 2 (MECP2) is a ~52 kDa protein. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-MECP2 Antibody (N227/12)

References

Datasheet

Anti-MECP2 Recombinant Antibody (N227/12).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MECP2 Recombinant Antibody (N227/12) (HW353023)

Overview

Images

References

Datasheet

SDS