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Anti-Mitofusin-1 Recombinant Antibody (N111/27) (HA938023)

Anti-Mitofusin-1 Recombinant Antibody (N111/27)
Anti-Mitofusin-1 Recombinant Antibody (N111/27)
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Overview
Catalog No.HA938023
Description
Anti-Mitofusin-1 Recombinant Antibody (N111/27) (HA938023) is a mouse monoclonal antibody detecting MFN1 in IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
ApplicationsIHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDN111/27
Target Fzo homolog, Transmembrane GTPase MFN1, MFN1, Mitofusin-1
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
RRID

Anti-Mitofusin-1 Antibody (N111/27) (abinScience Cat# HA938023, RRID:AB_3728536)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Mitofusin-1 (MFN1) is a ~84 kDa protein. Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro).

1. Legros, F. et al. (2002) Molecular biology of the cell 13, 4343-54. PMID: 12475957
2. Santel, A. et al. (2003) Journal of cell science 116, 2763-74. PMID: 12759376
3. Qi, Y. et al. (2016) The Journal of cell biology 215, 621-629. PMID: 27920125
4. Cao, YL. et al. (2017) Nature 542, 372-376. PMID: 28114303
Note For research use only.
Images
  • Anti-Mitofusin-1 Recombinant Antibody (N111/27)

    SDS-PAGE

    SDS-PAGE for Anti-Mitofusin-1 Antibody (N111/27)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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