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Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody (VK469014)

Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody
Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody
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  • 50ug
  • 100ug
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Overview
Catalog No.VK469014
Description
Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody (VK469014) is a rabbit polyclonal antibody detecting A33R in ELISA, IHC, WB. Suitable for Monkeypox virus, Vaccinia virus, and Variola virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMonkeypox virus (MPXV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Monkeypox virus/MPXV A33R (Met1-Asn113).
Target A33R
Purification Purified by antigen affinity column.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein OPG161 (A33R) is a ~20 kDa protein. Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.

1. Wolffe, EJ. et al. (2001) Journal of virology 75, 303-10. PMID: 11119600
2. Breiman, A. et al. (2013) The Journal of general virology 94, 720-725. PMID: 23255618
3. Monticelli, SR. et al. (2020) Journal of virology 94. PMID: 31941777
Note For research use only.
Images
  • Anti-Monkeypox virus/MPXV A33R Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-A33R polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Monkeypox virus/MPXV A33R (Catalog No: VK469012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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