Anti-Mouse ANG Polyclonal Antibody (MY052014)

Name: Anti-Mouse ANG Polyclonal Antibody
Brand: abinScience
SKU: MY052014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

MY052014

Description

Anti-Mouse ANG Polyclonal Antibody (MY052014) is a rabbit polyclonal antibody detecting Angiogenin; 3.1.27.-; Angiogenin-1; Ribonuclease 5; RNase 5; Ang; Ang1; Rnase5; Rnase5a in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mouse ANG (Gln25-Leu145).

Target

Angiogenin; 3.1.27.-; Angiogenin-1; Ribonuclease 5; RNase 5; Ang; Ang1; Rnase5; Rnase5a

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P21570

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36.

1. Fu, H. et al. (2009) FEBS letters 583, 437-42. PMID: 19114040

2. Goncalves, KA. et al. (2016) Cell 166, 894-906. PMID: 27518564

3. Bai, R. et al. (2020) The EMBO journal 39, e103325. PMID: 32510170

4. Liu, X. et al. (2021) Nature communications 12, 1832. PMID: 33758201

5. Yue, T. et al. (2021) Science (New York, N.Y.) 372. PMID: 33986151

6. Yu, W. et al. (2017) Cell 171, 849-864.e25. PMID: 29100074

7. Saikia, M. et al. (2014) Molecular and cellular biology 34, 2450-63. PMID: 24752898

Note

For research use only

Images

References

Datasheet

Anti-Mouse ANG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Mouse ANG Polyclonal Antibody (MY052014)

Overview

Images

References

Datasheet

SDS

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