Mouse ANG Recombinant Protein (N-His) (MY052012)

Name: Mouse ANG Recombinant Protein (N-His)
Brand: abinScience
SKU: MY052012
Price: 211 USD
Availability: InStock

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Catalog No.	MY052012
Description	Recombinant Mouse ANG Protein, N-His (MY052012) expressed in E. coli, spanning Gln25-Leu145. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P21570
Protein length	Gln25-Leu145
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.06 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Angiogenin; 3.1.27.-; Angiogenin-1; Ribonuclease 5; RNase 5; Ang; Ang1; Rnase5; Rnase5a
Background	Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36. 1. Fu, H. et al. (2009) FEBS letters 583, 437-42. PMID: 19114040 2. Goncalves, KA. et al. (2016) Cell 166, 894-906. PMID: 27518564 3. Bai, R. et al. (2020) The EMBO journal 39, e103325. PMID: 32510170 4. Liu, X. et al. (2021) Nature communications 12, 1832. PMID: 33758201 5. Yue, T. et al. (2021) Science (New York, N.Y.) 372. PMID: 33986151 6. Yu, W. et al. (2017) Cell 171, 849-864.e25. PMID: 29100074 7. Saikia, M. et al. (2014) Molecular and cellular biology 34, 2450-63. PMID: 24752898
Note	For research use only

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SDS-PAGE for Recombinant Mouse ANG Protein, N-His

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Mouse ANG Recombinant Protein (N-His).pdf

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Mouse ANG Recombinant Protein (N-His) (MY052012)

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