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Anti-Mouse IL-4R alpha Polyclonal Antibody (MB732014)

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Overview
Catalog No.MB732014
Description
Anti-Mouse CD124/IL4R/IL-4Rα Polyclonal Antibody (MB732014) is a rabbit polyclonal antibody detecting IL-4R-alpha in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse CD124/IL4R/IL-4Rα (Glu31-His225).
Target CD124, IL-4 receptor subunit alpha, IL-4-binding protein, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, IL4-BP, IL4R, IL4RA, Interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, Soluble interleukin-4 receptor subunit alpha, sIL4Ralpha/prot
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P16382
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Interleukin-4 receptor subunit alpha (CD124/IL4R) is a ~87 kDa protein. Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. CD124 is the therapeutic target of dupilumab (Dupixent).

Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse IL-4R alpha Polyclonal Antibody

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