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Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312) (MC336023)

Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)
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Overview
Catalog No.MC336023
Description
Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312) (MC336023) is a mouse monoclonal antibody detecting 4-1BB/TNFRSF9/CD137 in ELISA, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSAA2312
Tested applications WB
Target 4-1BB ligand receptor, CD137, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, Tumor necrosis factor receptor superfamily member 9
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P20334
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tumor necrosis factor receptor superfamily member 9 (CD137/4-1BB) is a ~27 kDa protein. Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors.

Note For research use only
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)

    Western blot

    Western blot analysis was performed using anti-CD137 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant mouse CD137 (Catalog No: MC336012)
    Lane 2: negative control

  • Anti-Mouse 4-1BB/TNFRSF9/CD137 Recombinant Antibody (SAA2312)

    Immunofluorescence

    Immunofluorescence staining of HDLM2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cell membrane.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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