Anti-Mouse SIRP alpha/CD172a Polyclonal Antibody (MX892014)

Name: Anti-Mouse SIRP alpha/CD172a Polyclonal Antibody
Brand: abinScience
SKU: MX892014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MX892014

Description

Anti-Mouse CD172a/SIRPA Polyclonal Antibody (MX892014) is a rabbit polyclonal antibody detecting CD172a in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Mouse CD172a/SIRPA (Gly31-Gly350).

Tested applications

Target

BIT, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P97797

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tyrosine-protein phosphatase non-receptor type substrate 1 (CD172a/SIRPA) is a ~56 kDa protein. Immunoglobulin-like cell surface receptor for CD47. Acts as a docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin.

1. Nakaishi, A. et al. (2008) Journal of molecular biology 375, 650-60. PMID: 18045614

2. Sarute, N. et al. (2019) PLoS biology 17, e3000137. PMID: 30726215

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SIRPA polyclonal antibody at SIRPA on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse cerebellum lysate
Lane 3: Rat brain lysate

References

Datasheet

Anti-Mouse SIRP alpha/CD172a Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse SIRP alpha/CD172a Polyclonal Antibody (MX892014)

Overview

Images

References

Datasheet

SDS

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