Anti-Mouse CD226/DNAM-1 Polyclonal Antibody (MS737014)

Name: Anti-Mouse CD226/DNAM-1 Polyclonal Antibody
Brand: abinScience
SKU: MS737014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

MS737014

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD226/DNAM-1 (Thr26-Pro254).

Target

CD226 antigen (Platelet and T-cell activation antigen 1) (CD antigen CD226)

Purification

Purified by antigen affinity column.

Accession

Q8K4F0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.

1. Iguchi-Manaka, A. et al. (2008) The Journal of experimental medicine 205, 2959-64. PMID: 19029379

2. Chan, CJ. et al. (2014) Nature immunology 15, 431-8. PMID: 24658051

3. Zhang, Z. et al. (2015) The Journal of experimental medicine 212, 2165-82. PMID: 26552706

4. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CD226/DNAM-1 polyclonal antibody at 1ug/mL on recombinant Mouse CD226/DNAM-1 (Catalog No. MS737012).

References

Datasheet

Anti-Mouse CD226/DNAM-1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD226/DNAM-1 Polyclonal Antibody (MS737014)

Overview

Images

References

Datasheet

SDS

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