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Anti-Mouse CD276/B7-H3 Polyclonal Antibody (MV612014)

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Anti-Mouse CD276/B7-H3 Polyclonal Antibody
Anti-Mouse CD276/B7-H3 Polyclonal Antibody
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Overview
Catalog No.MV612014
Description
Anti-Mouse CD276/B7-H3 Polyclonal Antibody (MV612014) is a rabbit polyclonal antibody detecting CD276/B7-H3 in ELISA, IHC, WB. Suitable for Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mouse CD276/B7-H3 (Thr26-Gly239).
Tested applications WB
Target 4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q8VE98
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

CD276 antigen (B7-H3) is a ~34 kDa protein. Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. CD276 is the therapeutic target of omburtamab (clinical).

Note For research use only.
Images
  • Anti-Mouse CD276/B7-H3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CD276 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: 293T cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse CD276/B7-H3 Polyclonal Antibody

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