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Anti-Mouse CD31/PECAM1 Polyclonal Antibody (MB004014)

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Overview
Catalog No.MB004014
Description
Anti-Mouse CD31/PECAM1 Polyclonal Antibody (MB004014) is a rabbit polyclonal antibody detecting PECAM1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CD31/PECAM1 (Ser21-Ser221).
Target PECAM-1, Platelet endothelial cell adhesion molecule, GPIIA', PECAM1, PECA1, EndoCAM, CD31
Purification Purified by antigen affinity column.
Accession Q08481
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a ~81 kDa protein. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal.

1. Stevens, HY. et al. (2008) Disease models & mechanisms 1, 175-81; discussion 179. PMID: 19048083
Note For research use only.
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Formula
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Formula
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Anti-Mouse CD31/PECAM1 Polyclonal Antibody

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