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Anti-Mouse CIDEC Polyclonal Antibody (MV727014)

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Overview
Catalog No.MV727014
Description
Anti-Mouse CIDEC Polyclonal Antibody (MV727014) is a rabbit polyclonal antibody detecting Cidec in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CIDEC (Arg41-Cys178).
Target Lipid transferase CIDEC, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27, Cidec, Fsp27
Purification Purified by antigen affinity column.
Accession P56198
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lipid transferase CIDEC is a ~27 kDa protein. Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage. Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1.

1. Keller, P. et al. (2008) The Journal of biological chemistry 283, 14355-65. PMID: 18334488
2. Gong, J. et al. (2011) The Journal of cell biology 195, 953-63. PMID: 22144693
3. Xu, W. et al. (2016) The Journal of biological chemistry 291, 4282-93. PMID: 26733203
4. Wang, J. et al. (2018) The Journal of biological chemistry 293, 19330-19343. PMID: 30361435
5. Qian, K. et al. (2023) Nature 613, 160-168. PMID: 36477540
6. Toh, SY. et al. (2008) PloS one 3, e2890. PMID: 18682832
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse CIDEC Polyclonal Antibody

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