Mouse CIDEC Recombinant Protein (N-His) (MV727012)

Name: Mouse CIDEC Recombinant Protein (N-His)
Brand: abinScience
SKU: MV727012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MV727012
Description	Recombinant Mouse CIDEC Protein, N-His (MV727012) expressed in E. coli, spanning Arg41-Cys178. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P56198
Protein length	Arg41-Cys178
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.23 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Lipid transferase CIDEC, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27, Cidec, Fsp27
Background	Lipid transferase CIDEC is a ~27 kDa protein. Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion. Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage. Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. 1. Keller, P. et al. (2008) The Journal of biological chemistry 283, 14355-65. PMID: 18334488 2. Gong, J. et al. (2011) The Journal of cell biology 195, 953-63. PMID: 22144693 3. Xu, W. et al. (2016) The Journal of biological chemistry 291, 4282-93. PMID: 26733203 4. Wang, J. et al. (2018) The Journal of biological chemistry 293, 19330-19343. PMID: 30361435 5. Qian, K. et al. (2023) Nature 613, 160-168. PMID: 36477540 6. Toh, SY. et al. (2008) PloS one 3, e2890. PMID: 18682832
Note	For research use only.

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SDS-PAGE for Recombinant Mouse CIDEC Protein, N-His

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Datasheet

Mouse CIDEC Recombinant Protein (N-His).pdf

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Mouse CIDEC Recombinant Protein (N-His) (MV727012)

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