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Anti-Mouse DAND5 Polyclonal Antibody (MA332014)

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Anti-Mouse DAND5 Polyclonal Antibody
Anti-Mouse DAND5 Polyclonal Antibody
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Overview
Catalog No.MA332014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse DAND5 (Ser55-Leu185).
Target DAN domain family member 5 (Cerberus-like protein 2) (Cerl-2)
Purification Purified by antigen affinity column.
Accession Q76LW6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DAN domain family member 5 (DAND5) is a ~19 kDa protein. Antagonist of the extracellular signaling protein NODAL, which is required for correct left-right patterning during embryonic development. Antagonist of BMP4 signaling. Antagonist of TGF-beta signaling. Independently of its role in left-right axis establishment, plays a role during heart development, possibly through the regulation of TGF-beta/Nodal signaling pathway. Displays anti-angiogenic activity by inhibiting endothelial sprouting, migration, and proliferation.

1. Marques, S. et al. (2004) Genes & development 18, 2342-7. PMID: 15466485
2. Araújo, AC. et al. (2014) PloS one 9, e102716. PMID: 25033293
3. Popovic, N. et al. (2021) EMBO molecular medicine 13, e12005. PMID: 33587337
Note For research use only.
Images
  • Anti-Mouse DAND5 Polyclonal Antibody

    Western blot

    Lane 1: MCF-7 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse DAND5 Polyclonal Antibody

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