Mouse DAND5 Recombinant Protein (N-GST & C-His) (MA332012)

Name: Mouse DAND5 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: MA332012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MA332012
Description	Recombinant Mouse DAND5 Protein, N-GST & C-His (MA332012) expressed in E. coli, spanning Ser55-Leu185. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q76LW6
Protein length	Ser55-Leu185
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.40 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DAN domain family member 5, Cerberus-like protein 2, Dand5, Cerl-2, Cerl2, Sp1
Background	DAN domain family member 5 (DAND5) is a ~19 kDa protein. Antagonist of the extracellular signaling protein NODAL, which is required for correct left-right patterning during embryonic development. Antagonist of BMP4 signaling. Antagonist of TGF-beta signaling. Independently of its role in left-right axis establishment, plays a role during heart development, possibly through the regulation of TGF-beta/Nodal signaling pathway. Displays anti-angiogenic activity by inhibiting endothelial sprouting, migration, and proliferation. 1. Marques, S. et al. (2004) Genes & development 18, 2342-7. PMID: 15466485 2. Araújo, AC. et al. (2014) PloS one 9, e102716. PMID: 25033293 3. Popovic, N. et al. (2021) EMBO molecular medicine 13, e12005. PMID: 33587337
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse DAND5 Protein, N-GST & C-His

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Datasheet

Mouse DAND5 Recombinant Protein (N-GST & C-His).pdf

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Mouse DAND5 Recombinant Protein (N-GST & C-His) (MA332012)

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