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Anti-Mouse EFNA1 Polyclonal Antibody (MB164014)

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Overview
Catalog No.MB164014
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse EFNA1 (Met1-Ser182).
Target Ephrin-A1 (EPH-related receptor tyrosine kinase ligand 1) (LERK-1) (Immediate early response protein B61) [Cleaved into: Ephrin-A1, secreted form]
Purification Purified by antigen affinity column.
Accession P52793
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ephrin-A1 (EFNA1) is a ~23 kDa protein. Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2. Acts as a ligand for EPHA3, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development. May also contribute to dendritic spine morphogenesis.

1. Hunter, SG. et al. (2006) Molecular and cellular biology 26, 4830-42. PMID: 16782872
2. Fang, WB. et al. (2008) The Journal of biological chemistry 283, 16017-26. PMID: 18387945
3. Frieden, LA. et al. (2010) Developmental dynamics : an official publication of the American Association of Anatomists 239, 3226-34. PMID: 20960543
4. Fu, WY. et al. (2007) Nature neuroscience 10, 67-76. PMID: 17143272
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse EFNA1 Polyclonal Antibody

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