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Mouse EFNA1 Recombinant Protein (C-Fc) (MB164021)

Mouse EFNA1 Recombinant Protein (C-Fc)
Mouse EFNA1 Recombinant Protein (C-Fc)
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Overview
Catalog No.MB164021
Description
Recombinant Mouse EFNA1 Protein, C-Fc (MB164021) expressed in Mammalian Cells, spanning Ala18-Ser182.
Highlights
  • Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • Mammalian Expression — Proper folding and native glycosylation.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian cells
AccessionP52793
Protein lengthAla18-Ser182
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Predicted molecular weight 47.76 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEphrin-A1, EPH-related receptor tyrosine kinase ligand 1, LERK-1, Immediate early response protein B61, Ephrin-A1 secreted form, Efna1, Epgl1, Epl1, Lerk1
Background

Ephrin-A1 is a ~23 kDa protein. Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2. Acts as a ligand for EPHA3, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development. May also contribute to dendritic spine morphogenesis.

1. Hunter, SG. et al. (2006) Molecular and cellular biology 26, 4830-42. PMID: 16782872
2. Fang, WB. et al. (2008) The Journal of biological chemistry 283, 16017-26. PMID: 18387945
3. Frieden, LA. et al. (2010) Developmental dynamics : an official publication of the American Association of Anatomists 239, 3226-34. PMID: 20960543
4. Fu, WY. et al. (2007) Nature neuroscience 10, 67-76. PMID: 17143272
Note For research use only
Images
  • Mouse EFNA1 Recombinant Protein (C-Fc)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse EFNA1 Protein, C-Fc

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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