Anti-Mouse EGFR Recombinant Antibody (7A7) (MF004013)

Name: Anti-Mouse EGFR Recombinant Antibody (7A7)
Brand: abinScience
SKU: MF004013
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MF004013

Species reactivity

Human, Mouse

Applications

ELISA

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

7A7

Target

EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q01279

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules.

1. Fazioli, F. et al. (1993) The EMBO journal 12, 3799-808. PMID: 8404850

2. Peng, SC. et al. (2010) Nucleic acids research 38, 7446-57. PMID: 20639532

3. Eagles, DA. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35131940

Note

For research use only

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Images

Bioactivity

Detects recombinant huamn EGFR (Catalog No: HF004011) and mouse EGFR (Catalog No: MF004011) in indirect ELISA

References

Datasheet

Anti-Mouse EGFR Recombinant Antibody (7A7).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse EGFR Recombinant Antibody (7A7) (MF004013)

Overview

Images

References

Datasheet

SDS

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