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Anti-Mouse ENPP1 Polyclonal Antibody (MB920014)

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Overview
Catalog No.MB920014
Description
Anti-Mouse ENPP1 Polyclonal Antibody (MB920014) is a rabbit polyclonal antibody detecting Enpp1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse ENPP1 (Lys617-Asp906).
Target Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Lymphocyte antigen 41, Ly-41, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, 3.1.4.1, Nucleotide pyrophosphatase, NPPase, 3.6.1.9, Nucleotide diphosphatase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form, Enpp1, Npps, Pc1, Pdnp1
Purification Purified by antigen affinity column.
Accession P06802
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) is a ~103 kDa protein. Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling; it is however unclear whether hedgehog inhibition is direct or indirect.

1. Johnson, K. et al. (1999) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 14, 883-92. PMID: 10352096
2. Johnson, KA. et al. (2000) American journal of physiology. Regulatory, integrative and comparative physiology 279, R1365-77. PMID: 11004006
3. Hessle, L. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 9445-9. PMID: 12082181
4. Bertrand, J. et al. (2012) Annals of the rheumatic diseases 71, 1249-53. PMID: 22510396
5. Hajjawi, MO. et al. (2014) Bone 69, 139-47. PMID: 25260930
6. Okawa, A. et al. (1998) Nature genetics 19, 271-3. PMID: 9662402
7. Babij, P. et al. (2009) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 24, 1552-64. PMID: 19419305
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse ENPP1 Polyclonal Antibody

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