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Anti-Mouse IL9 Polyclonal Antibody (MB771014)

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Overview
Catalog No.MB771014
Description
Anti-Mouse IL9 Polyclonal Antibody (MB771014) is a rabbit polyclonal antibody detecting Il9. Suitable for Mouse.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL9 (Gln19-Pro144).
Target Cytokine P40, IL-9, Il9, Interleukin-9, T-cell growth factor P40
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P15247
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Interleukin-9 (IL9) is a ~16 kDa protein. Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites. Affects intestinal epithelial permeability and adaptive immunity. In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs.

1. Townsend, JM. et al. (2000) Immunity 13, 573-83. PMID: 11070175
2. Elyaman, W. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 12885-90. PMID: 19433802
3. Khan, WI. et al. (2003) Infection and immunity 71, 2430-8. PMID: 12704113
4. Druez, C. et al. (1990) Journal of immunology (Baltimore, Md. : 1950) 145, 2494-9. PMID: 2145361
5. Kimura, Y. et al. (1995) International immunology 7, 115-20. PMID: 7718508
6. Demoulin, JB. et al. (1999) The Journal of biological chemistry 274, 25855-61. PMID: 10464327
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Mouse IL9 Polyclonal Antibody

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