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Anti-Mouse Kappa chain Recombinant Antibody (SAA0490) (MB980013)

Anti-Mouse Kappa chain Recombinant Antibody (SAA0490)
Anti-Mouse Kappa chain Recombinant Antibody (SAA0490)
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Overview
Catalog No.MB980013
Description
Anti-Mouse Kappa chain Recombinant Antibody (SAA0490) (MB980013) is a human monoclonal antibody detecting Kappa chain in ELISA, IHC, IF, IP, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, IF, IP, WB
Host speciesHuman
ClonalityMonoclonal
IsotypeVHH-Fc
Clone IDSAA0490
Target Immunoglobulin kappa constant, Ig kappa chain C region MOPC 21, Igkc
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Immunoglobulin kappa constant (Kappa Chain) is a ~11 kDa protein. Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
Note For research use only.
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  • Anti-Mouse Kappa chain Recombinant Antibody (SAA0490)

    SDS-PAGE

    SDS-PAGE for Anti-Mouse Kappa chain Antibody (SAA0490)

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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