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Anti-Mouse VISTA/B7-H5/PD-1H Polyclonal Antibody (MP543014)

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Overview
Catalog No.MP543014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse VSIR/VISTA (Ala32-Asn181).
Target V-type immunoglobulin domain-containing suppressor of T-cell activation (Platelet receptor Gi24) (V-set domain-containing immunoregulatory receptor) (V-set immunoregulatory receptor)
Purification Purified by antigen affinity column.
Accession Q9D659
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation.

1. Wang, L. et al. (2011) The Journal of experimental medicine 208, 577-92. PMID: 21383057
2. Flies, DB. et al. (2014) The Journal of clinical investigation 124, 1966-75. PMID: 24743150
3. Wang, L. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 14846-51. PMID: 25267631
4. Aloia, L. et al. (2010) The Journal of biological chemistry 285, 7776-83. PMID: 20042595
Note For research use only.
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Anti-Mouse VISTA/B7-H5/PD-1H Polyclonal Antibody

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