Anti-MRE11 Polyclonal Antibody (HW535014)

Name: Anti-MRE11 Polyclonal Antibody
Brand: abinScience
SKU: HW535014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW535014

Description

Anti-MRE11 Polyclonal Antibody (HW535014) is a rabbit polyclonal antibody detecting MRE11 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MRE11 (Pro375-Lys609).

Target

HNGS1, Double-strand break repair protein MRE11, MRE11A, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog A, MRE11 homolog A, MRE11 homolog 1, MRE11, Meiotic recombination 11 homolog 1

Purification

Purified by antigen affinity column.

Accession

P49959

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Double-strand break repair protein MRE11 is a ~80 kDa protein. Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection.

1. de Jager, M. et al. (2001) Molecular cell 8, 1129-35. PMID: 11741547

2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032

3. Park, YB. et al. (2011) Structure (London, England : 1993) 19, 1591-602. PMID: 22078559

4. Limbo, O. et al. (2012) Nucleic acids research 40, 11435-49. PMID: 23080121

5. Shibata, A. et al. (2014) Molecular cell 53, 7-18. PMID: 24316220

6. Kijas, AW. et al. (2015) Nucleic acids research 43, 8352-67. PMID: 26240375

7. Anand, R. et al. (2016) Molecular cell 64, 940-950. PMID: 27889449

8. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292

9. Coquel, F. et al. (2018) Nature 557, 57-61. PMID: 29670289

10. He, YJ. et al. (2018) Nature 563, 522-526. PMID: 30464262

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MRE11 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate