Recombinant Human MRE11 Protein, C-His (HW535011) expressed in Insect Cells, spanning Met1-Arg708. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Insect cells
Accession
P49959
Protein length
Met1-Arg708
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
81.56 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
HNGS1, Double-strand break repair protein MRE11, MRE11A, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog A, MRE11 homolog A, MRE11 homolog 1, MRE11, Meiotic recombination 11 homolog 1
Background
Double-strand break repair protein MRE11 is a ~80 kDa protein. Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection.
1. de Jager, M. et al. (2001) Molecular cell 8, 1129-35. PMID: 11741547
2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032
3. Park, YB. et al. (2011) Structure (London, England : 1993) 19, 1591-602. PMID: 22078559
4. Limbo, O. et al. (2012) Nucleic acids research 40, 11435-49. PMID: 23080121
5. Shibata, A. et al. (2014) Molecular cell 53, 7-18. PMID: 24316220
6. Kijas, AW. et al. (2015) Nucleic acids research 43, 8352-67. PMID: 26240375
7. Anand, R. et al. (2016) Molecular cell 64, 940-950. PMID: 27889449
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