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Anti-Mycoplasma pneumoniae P41 Polyclonal Antibody (JN180034)

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Overview
Catalog No.JN180034
Description
Anti-Mycoplasma pneumoniae Wheel complex protein P41 Polyclonal Antibody (JN180034) is a rabbit polyclonal antibody detecting Wheel complex protein P41. Suitable for Mycoplasma pneumoniae.
Species reactivityMycoplasma pneumoniae
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Mycoplasma pneumoniae Wheel complex protein P41 (Met1-Gln220).
Target Cytoskeletal protein P41, Wheel complex protein P41
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P75470
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Wheel complex protein P41 is a ~40 kDa protein. Stably anchors the attachment organelle to the cell body and for their spatial positioning. Required for stable accumulation of P28 (also called HMW2-s, a short protein produced by upstream MPN_310). Required for correct localization of P24. Ectopic expression of P41 restores gliding velocity but not gliding frequency to a P41-P24 disruption, attachment organelles do not initiate as frequently as in wild-type; inclusion of P24 restores a wild-type phenotype. May play a role in attachment organelle initiation and its coordination with the cell cycle (Probable).

1. Hasselbring, BM. et al. (2007) Molecular microbiology 63, 44-53. PMID: 17163973
2. Hasselbring, BM. et al. (2007) Journal of bacteriology 189, 7442-9. PMID: 17693502
3. Nakane, D. et al. (2015) PLoS pathogens 11, e1005299. PMID: 26633540
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mycoplasma pneumoniae P41 Polyclonal Antibody

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