

| Catalog No. | JN180032 |
|---|---|
| Description |
Recombinant Mycoplasma pneumoniae Wheel complex protein P41, N-His (JN180032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | P75470 |
| Protein length | Met1-Gln220 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Mycoplasma pneumoniae |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Cytoskeletal protein P41, Wheel complex protein P41 |
| Background | Wheel complex protein P41 is a ~40 kDa protein. Stably anchors the attachment organelle to the cell body and for their spatial positioning. Required for stable accumulation of P28 (also called HMW2-s, a short protein produced by upstream MPN_310). Required for correct localization of P24. Ectopic expression of P41 restores gliding velocity but not gliding frequency to a P41-P24 disruption, attachment organelles do not initiate as frequently as in wild-type; inclusion of P24 restores a wild-type phenotype. May play a role in attachment organelle initiation and its coordination with the cell cycle (Probable). 1. Hasselbring, BM. et al. (2007) Molecular microbiology 63, 44-53. PMID: 17163973 2. Hasselbring, BM. et al. (2007) Journal of bacteriology 189, 7442-9. PMID: 17693502 3. Nakane, D. et al. (2015) PLoS pathogens 11, e1005299. PMID: 26633540 |
| Note | For research use only |
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