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Mycoplasma pneumoniae Wheel complex P41 Recombinant Protein (N-His) (JN180032)

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Overview
Catalog No.JN180032
Description
Recombinant Mycoplasma pneumoniae Wheel complex protein P41, N-His (JN180032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP75470
Protein lengthMet1-Gln220
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMycoplasma pneumoniae
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCytoskeletal protein P41, Wheel complex protein P41
Background

Wheel complex protein P41 is a ~40 kDa protein. Stably anchors the attachment organelle to the cell body and for their spatial positioning. Required for stable accumulation of P28 (also called HMW2-s, a short protein produced by upstream MPN_310). Required for correct localization of P24. Ectopic expression of P41 restores gliding velocity but not gliding frequency to a P41-P24 disruption, attachment organelles do not initiate as frequently as in wild-type; inclusion of P24 restores a wild-type phenotype. May play a role in attachment organelle initiation and its coordination with the cell cycle (Probable).

1. Hasselbring, BM. et al. (2007) Molecular microbiology 63, 44-53. PMID: 17163973
2. Hasselbring, BM. et al. (2007) Journal of bacteriology 189, 7442-9. PMID: 17693502
3. Nakane, D. et al. (2015) PLoS pathogens 11, e1005299. PMID: 26633540
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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