Anti-MYL2 Polyclonal Antibody (HY376014)

Name: Anti-MYL2 Polyclonal Antibody
Brand: abinScience
SKU: HY376014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY376014

Description

Anti-MYL2 Polyclonal Antibody (HY376014) is a rabbit polyclonal antibody detecting MYL2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MYL2 (Ala2-Ile159).

Target

Ventricular myosin light chain 2, Myosin light chain 2, slow skeletal/ventricular muscle isoform, Cardiac myosin light chain 2, MLC-2v, MLC-2s/v, MLC-2, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC2, MYL2

Purification

Purified by antigen affinity column.

Accession

P10916

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MYL2) is a ~18 kDa protein. Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.

1. Weterman, MA. et al. (2013) Brain : a journal of neurology 136, 282-93. PMID: 23365102

2. Manivannan, SN. et al. (2020) PLoS genetics 16, e1008639. PMID: 32453731

Note

For research use only.

Images

Western blot

Lane 1: Mouse heart lysate
Lane 2: Mouse skeletal muscle lysate
Lane 3: Rat heart lysate

References

Datasheet

Anti-MYL2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MYL2 Polyclonal Antibody (HY376014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us