Human MYL2 Recombinant Protein (N-His) (HY376012)

Name: Human MYL2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY376012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY376012
Description	Recombinant Human MYL2 Protein, N-His (HY376012) expressed in E. coli, spanning Ala2-Ile159. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P10916
Protein length	Ala2-Ile159
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.15 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ventricular myosin light chain 2, Myosin light chain 2, slow skeletal/ventricular muscle isoform, Cardiac myosin light chain 2, MLC-2v, MLC-2s/v, MLC-2, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC2, MYL2
Background	Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MYL2) is a ~18 kDa protein. Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly. 1. Weterman, MA. et al. (2013) Brain : a journal of neurology 136, 282-93. PMID: 23365102 2. Manivannan, SN. et al. (2020) PLoS genetics 16, e1008639. PMID: 32453731
Note	For research use only.

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SDS-PAGE for Recombinant Human MYL2 Protein, N-His

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Datasheet

Human MYL2 Recombinant Protein (N-His).pdf

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Human MYL2 Recombinant Protein (N-His) (HY376012)

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