Anti-NKCC1 Recombinant Antibody (N161/1) (HX014013)

Name: Anti-NKCC1 Recombinant Antibody (N161/1)
Brand: abinScience
SKU: HX014013
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX014013

Description

Anti-NKCC1 Recombinant Antibody (N161/1) (HX014013) is a mouse monoclonal antibody detecting SLC12A2/NKCC1 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N161/1

Target

SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Solute carrier family 12 member 2, NKCC1, Basolateral Na-K-Cl symporter

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-NKCC1 Antibody (N161/1) (abinScience Cat# HX014013, RRID:AB_3728281)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Solute carrier family 12 member 2 (SLC12A2/NKCC1) is a ~131 kDa protein. Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.

1. Vitari, AC. et al. (2006) The Biochemical journal 397, 223-31. PMID: 16669787

2. Yang, X. et al. (2020) Nature communications 11, 1016. PMID: 32081947

3. Mutai, H. et al. (2020) PLoS genetics 16, e1008643. PMID: 32294086

4. Zhang, S. et al. (2021) Communications biology 4, 226. PMID: 33597714

5. Zhao, Y. et al. (2022) Nature communications 13, 2747. PMID: 35585053

6. Neumann, C. et al. (2022) The EMBO journal 41, e110169. PMID: 36239040

7. Moseng, MA. et al. (2022) Science advances 8, eabq0952. PMID: 36306358

8. Payne, JA. et al. (1995) The Journal of biological chemistry 270, 17977-85. PMID: 7629105

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-NKCC1 Antibody (N161/1)

References

Datasheet

Anti-NKCC1 Recombinant Antibody (N161/1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NKCC1 Recombinant Antibody (N161/1) (HX014013)

Overview

Images

References

Datasheet

SDS